nucleotide sequencing

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• Sequencing by ligation — is a DNA sequencing method that uses the enzyme DNA ligase to identify the nucleotide present at a given position in a DNA sequence. Unlike most currently popular DNA sequencing methods, this method does not use a DNA polymerase to create a… …   Wikipedia

• Sequencing — For the sense of sequencing used in electronic music, see the music sequencer article. For sequence learning in cognitive science, see sequence learning. In genetics and biochemistry, sequencing means to determine the primary structure (sometimes …   Wikipedia

• DNA sequencing — Part of a series on Genetics Key components Chromosome DNA • RNA Genome Heredity Mutation Nucleotide Variation …   Wikipedia

• Bisulfite sequencing — Figure 1: Outline of bisulfite conversion of sample sequence of genomic DNA. Nucleotides in blue are unmethylated cytosines converted to uracils by bisulfite, while red nucleotides are 5 methylcytosines resistant to conversion …   Wikipedia

• DNA nanoball sequencing — is a high throughput sequencing technology that is used to determine the entire genomic sequence of an organism. The method uses rolling circle replication to amplify small fragments of genomic DNA into DNA nanoballs. Fluorescent probes bind to… …   Wikipedia

• Single Molecule Real Time Sequencing — (also known as SMRTTM) is a parallelized single molecule DNA sequencing by synthesis developed by [http://www.pacificbiosciences.com/index.php Pacific BiosciencesTM] . Pacific Biosciences is a company based in Menlo Park, California, which was… …   Wikipedia

• Nanopore sequencing — is a method under development since 1995 [1][2] for determining the order in which nucleotides occur on a strand of DNA. A nanopore is simply a small hole, of the order of 1 nanometer in internal diameter. Certain transmembrane cellular proteins… …   Wikipedia

• Massive parallel sequencing — is a term used to describe several revolutionary approaches to DNA sequencing, the so called next generation sequencing (NGS) technologies or second generation sequencing. These sequencing technologies have emerged in late 1996 [1][2] an have… …   Wikipedia

• Microfluidic Sanger sequencing — The completion of the Human Genome Project has been a cornerstone in the advancement of biological studies. The outcomes of obtaining a complete reference map (including the sequence) of the human genome have ushered in the post genome era of… …   Wikipedia

• ABI Solid Sequencing — is a form of DNA sequencing. DNA sequencing encompasses biochemical methods for determining the order of the nucleotide bases, adenine, guanine, cytosine, 5 methyl cytosine, and thymine, in a DNA oligonucleotide which is described by McKernan,… …   Wikipedia

• DNA sequencing — ▪ genetics  technique used to determine the nucleotide sequence of DNA (deoxyribonucleic acid). The nucleotide sequence is the most fundamental level of knowledge of a gene or genome. It is the blueprint that contains the instructions for… …   Universalium